The AOAC 991.48 standard, developed by the Official Society of Analytical Chemists (AOAC), describes a test method that involves testing disinfectants against the staphylococcus aureus bacterial strain.
This test method is a standard disinfectant test method developed for staphylococcus aureus cells in biofilms. This test method uses two disinfectants, the membrane active compound benzalkonium chloride and the oxidizing agent sodium hypochlorite, to evaluate the biofilm test. During the tests, biofilms are prepared on glass, stainless steel and polystyrene in a simple system with a constant nutrient flow, which closely mimics the conditions used in the European disinfectant testing standard EN 1040. The biofilm formed on the glass contains cell clumps and extracellular polysaccharides. The average surface coverage is 60 percent and most of the biofilm cells are viable. Biofilm formation and biofilm disinfection are repeated in different tests. For biofilms exposed to benzalkonium chloride and sodium hypochlorite, the concentrations required to achieve 4-log kill are many times higher than the concentrations required to reach the European level.
Foodborne diseases cause millions of diseases worldwide each year. One way food can be contaminated with pathogens is through contact with food processing equipment. It is therefore of the utmost importance that this equipment is regularly and adequately cleaned and disinfected. Businesses should use an effective disinfectant and this disinfectant should be in an appropriate concentration. A concentration that is too low increases the risk of food contamination and resistance to the disinfectant, while a concentration that is too high imposes a cost and environmental burden.
The procedure used to test disinfectants in European Union countries consists of three stages. In the first step, the basic activity of the product is tested with a suspension test. The second stage consists of two steps. In the first step, the product is tested with a suspension test under conditions representative of practical conditions, in the second step other laboratory tests are carried out (eg surface tests simulating practical conditions). The third stage consists of field tests..
Among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors, our organization also provides testing services against staphylococcus aureus bacteria species of disinfectants within the scope of AOAC 991.48 standard, with its trained and expert staff and advanced technological equipment.
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