ASTM E1153-14 Standard Test Method for Efficacy of Recommended Disinfectants for Inanimate, Hard, Non-Porous, Non-Food Contact Surfaces

The ASTM E1153-14 standard developed by the American Testing and Materials Organization (ASTM) is a standard for evaluating the antimicrobial efficacy of disinfectants against staphylococcus aureus or klebsiella pneumoniae or enterobacter aerogenes or a combination of these on pre-cleaned, lifeless, hard, non-porous, non-food contact surfaces. test method is presented. Appropriate modifications to the method may be required when testing organisms not listed here. Modifications may also be necessary when using test substrates not described here (test method described in the ASTM E2274 standard) or when evaluating spray-based or towel-based antimicrobial products.

This test method is also used to evaluate the antimicrobial efficacy of one-step cleaner / disinfectant formulations recommended for use on lightly soiled, inanimate, non-porous, non-food contact surfaces. It is the research assistant's responsibility to determine if Good Laboratory Practices (GLP) are required and to follow them where appropriate.

During the test, the surfaces are inoculated with 10 µl of staphylococcus aureus and klebsiella pneumoniae (or enterobacter aerogenes) and dried at 35 degrees Celsius for 35 minutes. The surfaces are transferred to sterile jars, petri plates or other suitable sterile containers using sterile forceps and covered with a predetermined volume of disinfectant product and held at ambient temperature for the desired contact time. After the exposure time, the appropriate neutralizing solution is added to the jar containing the exposed surface and vigorously rotated for approximately 50 turns to suspend any surviving organisms. Surviving bacteria are determined by the pour plate method.

There are multiple test checks in this method to ensure the validity of test results. Parallel controls measure the amount of bacteria removed from surfaces by application method (i.e. spraying or wiping). Neutralization controls confirm that the neutralizing recovery method stops the antimicrobial activity of the disinfectant product at the expiration of the contact time.

Our organization, among the numerous test, measurement, analysis and evaluation studies it has given for businesses in various sectors, with its trained and expert staff and advanced technological equipment, for the effectiveness of disinfectants recommended for inanimate, hard, non-porous, non-food contact surfaces within the scope of ASTM E1153-14 standard. It also provides standard testing services.