The ASTM E645-18 standard, developed by the American Society for Testing and Materials (ASTM), outlines an application for evaluating the effectiveness of microbicides (algicides, bactericides, and fungicides) used to control microbial growth in cooling water systems. Microbicides are evaluated using simulated or real cooling tower water against:
The practice defined in the ASTM E645-18 standard is to identify potentially effective microbicides for use in cooling water systems using field-derived coolant and deposits/biofilm. The addition of deposits / biofilms satisfies the need to include the main source of microorganisms in cooling water systems. With this addition, laboratory results may not fully predict microbicidal efficacy in the field. This is because the conditions in the field affecting microbicide activity are difficult to mimic in the laboratory.
The main conditions affecting microbicide efficacy are: rate of degradation, make-up water addition, water hardness, hydrocarbon seeps, pH value, sediment loading, dissolved solids, microbes (biofilms) and sediments (salts, iron minerals, organics) in the slime (slime). An additional factor is the difficulty of counting all microbes in the water due to the lack of adequate recovery media. An application that addresses the formation and testing of surface-attached microbes (biofilms) is described in the ASTM E1427 standard, and an application for non-traditional microbe measurement is described in the ASTM E1326 standard.
Briefly, during this test, microbicides are evaluated against water or sediment microbes under conditions that simulate a cooling water system. For this, microbicides in concentrations selected by the manufacturer are confronted with a cooling water or sediment suspension. After the selected contact time, the number of microbes (aerobic bacteria, anaerobic bacteria, fungi or algae) is determined and compared to the values at the beginning of the test without product exposure.
Our organization also provides standard testing services for the evaluation of microbicides used in cooling water systems within the scope of ASTM E645-18 standard, with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.
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