Size exclusion chromatography or size separation chromatography (SEC) is a separation technique based on the molecular size of components. Separation is achieved by differential exclusion of sample molecules from the pores of the packaging material as they pass through a bed of porous particles. The key feature of this assay is its gentle, non-absorbent interaction with the sample, which ensures a high level of biomolecular activity.
-analizleri.jpg "Size Exclusion Chromatography (SEC) Analyzes")
Size separation chromatography is a widely used chromatographic separation method for the characterization of synthetic polymers. In this method, the size-dependent pore accessibility of the polymer molecules is used and the polymer molecules are separated according to their chain sizes. Polymer chain size depends not only on molecular weight, but also on other molecular properties such as composition, chain architecture, and microstructure. Therefore, this method of analysis cannot, for example, separate nonlinear homopolymers by molecular weight or copolymers by composition.
Size exclusion chromatography separates molecules by size by filtration through a gel. The gel consists of spherical beads containing pores with a certain size distribution. Separation occurs when molecules of different sizes are introduced into or removed from the pores in the matrix.
A standard based on these analyzes is the International Standards Organization. It is the ISO 16014 standard developed by This standard consists of the following sections under the main heading "Plastics - Determination of the average molecular weight and molecular weight distribution of polymers using size exclusion chromatography":
This standard describes a general method for determining the average molecular weight and molecular weight distribution of polymers using size exclusion chromatography (SEC). Average molecular weight and molecular weight distribution can be obtained from a calibration curve constructed using polymer standards if using one of the methods described in sections two, three and four, or from a calibration curve generated using absolute molecular weight data if size exclusion chromatography combined with light scattering detection is used as described in section five. is calculated.
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