Determination of Klebsiella pneumoniae

Klebsiella pneumoniae is a pathogen commonly encountered in hospitals. In clinical context, it causes pneumonia, bronchitis, urinary tract and wound infections especially in infants, diabetics, cancer patients, antibiotic users and elderly people. In addition, antibiotic-resistant klebsiella pneumoniae, which has emerged in recent years, has become a serious problem in clinics. Therefore, rapid and sensitive detection of this pathogen is required if appropriate treatment is to be administered and outbreaks are to be controlled.

Klebsiella pneumoniae is one of the most problematic pathogens associated with antibiotic resistance worldwide. Misidentification is frequently made using standard laboratory methods, and as a result, the clinical significance of members of the klebsiella pneumoniae complex has not been definitively defined. Currently, most infections due to klebsiella pneumoniae in North American and European countries are due to classical klebsiella pneumoniae strains and occur mainly in hospitals and long-term care facilities. These strains are of increasing clinical importance because of their tendency to acquire plasmids containing multiple antimicrobial resistance markers, which complicates treatment.

Traditional methods for detecting Klebsiella pneumoniae include microscopic examination, biochemical identification, and the use of newly developed automated bacterial identification tools. However, these methods are time consuming, have low sensitivity and usually require several days of incubation time. Recently, a number of molecular biological techniques have been used to detect klebsiella pneumoniae. However, a variety of newly used PCR techniques are relatively complex and require specialized, expensive tools. Therefore, another fast, simple and cost-effective assay is being developed to complement existing PCR methods. For example, the loop-mediated isothermal amplification method is a new nucleic acid detection method based on DNA synthesis. This method has high specificity as it uses four or six specific primers that recognize six or eight different sequences on the target gene. This technique is widely used in the clinical detection of pathogens, including bacteria, viruses, parasites and fungi.

Our organization also provides test services for the determination of klebsiella pneumoniae with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.