HET-CAM is a very useful in vitro test method applied to evaluate the eye irritant properties of cosmetic formulations and ingredients. One of the most important biological properties of consumer products, as well as many raw materials, is local compatibility with mucous membranes. So far, standardized in vivo tests have been considered valid for detecting the irritation potential of chemicals and suitable for risk assessment. However, the debate about animal testing, and especially rabbit eye testing, is increasing in the public and scientific realm. One of the most important in vitro tests is the HET-CAM test method, which is the chicken egg test on the chorioallantoic membrane (CAM) of fertilized chicken eggs. This method has been pre-validated with approximately 100 test substances covering a wide range of chemical structures and physical appearances and representing the range of chemicals in the cosmetic industry. HET-CAM has proven to be a robust test with a good estimate of irritation potential.
Briefly, chicken egg chorioallantoic membrane test (HET-CAM test) is applied to determine the irritation potential of substances. In this test method, incubating chicken eggs are carefully opened on the ninth day and the chorioallantoic membrane (CAM) is exposed. Test samples, pure or diluted solutions, are placed directly on the exposed chorioallantoic membrane membrane and the membrane is visually inspected with a microscope. The reaction time on the membrane is 5 minutes. The results of the reaction are bleeding, coagulation and fragmentation. Based on the reaction time, an irritation score is calculated and the items are classified according to the score value as follows:
Data on eye irritation are often needed for hazard identification of chemicals. The chicken egg test - chorioallantoic membrane (HET-CAM) method is used for routine testing of a wide variety of chemicals and formulations. Since this test method has no global regulatory acceptance, rabbit eye irritation testing is also performed in accordance with OECD procedure 405.
Our organization also provides in vitro HET-CAM testing services with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.
To get an appointment, to get more detailed information or to request an evaluation, you can ask us to fill in our form and reach you.
EUROLAB reporting and certification activities, United Accreditation Foundation (UAF), Turkish Accreditation Agency (TÜRKAK), Enterprise Accreditation Foundation (EAF) based on the accreditation provided by its institution. The Enterprise Accreditation Foundation (EAF) is also a signatory to the United Nations (UN GLOBAL COMPACT).
