The AOAC 966.04 standard, developed by the Official Society of Analytical Chemists (AOAC), describes a test method that evaluates water-soluble powders and non-volatile liquid products for spore-killing activity. This test method is used for registration of disinfectants and verification of US Environmental Protection Agency (EPA) and US Food and Drug Administration (FDA) sporicidal efficacy claims.
In this test method Peni rollers and silk sutures are inoculated with bacillus subtilis and clostridium sporogenes. These carriers are exposed to the test substance for a predetermined time. Carriers are then incubated and examined for the presence or absence of growth of spores. For the test substance to act as a disinfectant, it must kill a predetermined number of test organisms on the carriers.
The AOAC 966.04 Sporicidal activity test is required to verify sporeicidal claims. The starting number of spores per carrier was determined to ensure a robust challenge for the disinfectant. Testing then begins with the transfer of dried, inoculated carriers to the test disinfectant. The carriers are exposed to the disinfectant for a specified contact time, then transferred to a recovery medium supplemented with a neutralization compound suitable for the active ingredient of the disinfectant. The recovery water allows spores not killed in the disinfection process to remain viable prior to incubation. An incubation period of 21 days gives the microbe a chance to grow to detectable levels for qualitative assessment of growth.
Hospital-acquired infections are caused by a variety of pathogens, including clostridium difficile. Patients at high risk are those who are hospitalized for a long time and the elderly. This bacterium can spread rapidly. Clostridium difficile produces resistant spores that are less sensitive to biocidal products than the vegetative form. These spores can remain on surfaces for a long time. A small number of clostridium difficile spores is sufficient to initiate an infection. Disease outbreaks associated with Clostridium difficile occur episodically in most healthcare settings.
Our organization provides sporicidal activity test services within the scope of AOAC 966.04 standard, with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors. also provides.
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