The ASTM E1054-21 standard, developed by the American Society for Testing and Materials (ASTM), describes applications for determining the effectiveness of methodology procedures and materials for inactivating (neutralizing, quenching) the microbicidal properties of antimicrobial agents.
These test procedures are used to:
Knowledge of microbiological and statistical techniques is required for these processes. These methods are not suitable when testing the virucidal activity of microbicides. ASTM E on thisThe 1482 standard must be complied with.
The effectiveness of antimicrobial agents added to disinfectants and antiseptics is measured by their ability to kill microorganisms within a certain contact time. Therefore, accurate determination of antimicrobial activity requires complete and rapid inactivation (neutralization) of the antimicrobial agent. Inadequate or incomplete neutralization allows killing or inactivation of microorganisms to continue beyond the experimental exposure time, resulting in an overestimation of antimicrobial activity.
Neutralization methods commonly used in antimicrobial efficacy evaluations are chemical inactivation, dilution and filtration. All critical parameters of an antimicrobial efficacy assessment, such as the environment, equipment, microorganisms, and temperature of the solutions, should be replicated in the performance of the neutralization procedure chosen.
At least three replicates should be performed before an analysis of the neutralization assessment and microbial recovery data can be performed. The number of replicates used in the assessment depends on the statistical significance required for expected results, the variability encountered in the data, and the relative effectiveness of the neutralization procedure.
Ideally, neutralization tests should be performed on all microorganisms used in the evaluation of antimicrobial efficacy. However, representative organisms may be selected for testing. The inability to determine neutralizing efficacy and toxicity for all microorganisms can be misleading in an antimicrobial efficacy assessment.
Among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors, our organization also provides test services for standard applications for the evaluation of inactivators of antimicrobial agents within the scope of ASTM E1054-21 standard, with its trained and expert staff and advanced technological equipment.
To get an appointment, to get more detailed information or to request an evaluation, you can ask us to fill in our form and reach you.
EUROLAB reporting and certification activities, United Accreditation Foundation (UAF), Turkish Accreditation Agency (TÜRKAK), Enterprise Accreditation Foundation (EAF) based on the accreditation provided by its institution. The Enterprise Accreditation Foundation (EAF) is also a signatory to the United Nations (UN GLOBAL COMPACT).
