Advanced laboratories perform an in vitro red blood cell test (RBC test) to estimate the irritation potential of detergents. The prediction in this test method is based on the differentiation between cell membrane lysis and cell protein denaturation. Both effects are measured photometrically using the natural dye oxyhemoglobin. This test method is an inexpensive, rapid irritant screening test that provides reliable results with good reproducibility. On the other hand, this testing method helps to reduce or even avoid animal testing.
The aforementioned red blood cell (RBC) test is presented as a biological in vitro test for rapid evaluation of the membrane and protein damaging effects of detergents, namely surfactants. The system uses hemoglobin released during red blood cell (RBC) damage as an indicator to quantify the concentration at which surfactants affect and disrupt the plasma membrane. The spectrophotometric assay also takes into account the denaturation of hemoglobin caused by high concentrations of detergents. Thus, a denaturation index is determined for each test substance, as well as the half-maximum concentrations that will cause hemolysis.
Meanwhile, in vivo toxicity tests have been conducted for years to assess the potential risks of numerous medications and cosmetic products used for the eye. These tests, when performed on animals, carry the potential to cause them significant discomfort and pain. Therefore, new alternative methods are being developed to determine the potential toxic effects of various chemical compounds and cosmetic products. Among these methods, in vitro red blood cell tests are widely applied. In fact, in European Union countries, testing personal care and cosmetic products on animals is prohibited, and alternative in vitro toxicity tests are used in the safety assessment of these products. In vitro reconstructed tissue models are used as an alternative method to determine the eye irritation potential of these products. Some of these models are very similar to the human eye in terms of morphology and physiology and are therefore preferred.
Our organization also provides in vitro red blood cell testing services with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.
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