In Vitro Red Blood Cell Test

Advanced laboratories perform an in vitro red blood cell test (RBC test) to estimate the irritation potential of detergents. The prediction in this test method is based on the differentiation between cell membrane lysis and cell protein denaturation. Both effects are measured photometrically using the natural dye oxyhemoglobin. This test method is an inexpensive, rapid irritant screening test that provides reliable results with good reproducibility. On the other hand, this testing method helps to reduce or even avoid animal testing.

The aforementioned red blood cell (RBC) test is presented as a biological in vitro test for rapid evaluation of the membrane and protein damaging effects of detergents, namely surfactants. The system uses hemoglobin released during red blood cell (RBC) damage as an indicator to quantify the concentration at which surfactants affect and disrupt the plasma membrane. The spectrophotometric assay also takes into account the denaturation of hemoglobin caused by high concentrations of detergents. Thus, a denaturation index is determined for each test substance, as well as the half-maximum concentrations that will cause hemolysis.

Meanwhile, in vivo toxicity tests have been carried out for years in order to evaluate the risk potential of many drugs and cosmetic products used for the eye. Performing these tests on animals has the potential to cause them serious discomfort and pain. In this regard, new alternative methods are being developed to determine the possible toxic effects of various chemical compounds and cosmetic products. Among these methods In vitro red blood cell tests are widely practiced. In fact, in the European Union countries, testing of personal care and cosmetic products on animals is prohibited and alternative in vitro toxicity tests are applied in the safety evaluations of the products. In vitro reconstructed tissue models are used as an alternative method to determine the eye irritation potential of these products. Some of these models are morphological and Physiologically it is very similar to the human eye and is therefore more preferred.

Our organization also provides in vitro red blood cell testing services with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.