The OECD Library is the online library of the Organization for Economic Co-operation and Development (OECD) books, articles and statistics, and is the knowledge base of its analysis and data. One of the test methods included here is the OECD 471 Bacterial reverse mutation test method.
The OECD guidelines for testing chemicals are a collection of approximately 150 internationally accepted test methods used by government agencies, industry businesses, and independent laboratories to identify and characterize the potential hazards of chemicals. These are a set of tools for professionals, primarily used in regulatory safety testing, and subsequently in chemical and chemical product notification, chemical registration, and chemical evaluation. They are also used during the development of new chemicals and products, and in toxicology research, for the selection and ranking of candidate chemicals. Collectively, all these test methods address health effects.
The OECD 471 Bacterial reverse mutation test uses amino acids that require at least five strains of salmonella and escherichia coli to detect point mutations with base substitutions or frameshifts. The principle of this bacterial reverse mutation test is to detect mutations that reverse the mutations found in the test strains and restore the functional capacity of the bacteria to synthesize an essential amino acid.
When performing the test, suspensions of bacterial cells are exposed to the liquid or solid test substance in the presence and absence of an exogenous metabolic activation system. At least five different assayable concentrations of the test substance should be used. The recommended maximum test concentration for soluble non-cytotoxic substances is 5 mg/plate or 5 ml/plate.
Two methods are applied during this test: the plate bonding method and the pre-incubation method. For both methods, after two or three days of incubation at 37 degrees Celsius, the returning colonies are counted and compared to the number of spontaneously returning colonies on the solvent control plates.
Among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors, our organization also provides bacterial reverse mutation testing services within the scope of OECD 471 standard, with its trained and expert staff and advanced technological equipment.
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