The ASTM E2562-17 standard published by the American Society for Testing and Materials (ASTM) describes the operational parameters required to grow 2 pseudomonas aeruginosa biofilms reproducibly under high shear. The resulting biofilm represents generalized situations where the biofilm exists under high shear rather than representing a specific environment.
This test method uses the Centers for Disease Control and Prevention (CDC) biofilm reactor. This reactor is a continuously stirred tank reactor with high wall shear. It was originally designed to model a drinking water system for the evaluation of the bacterial strain Legionella pneumophila. However, this reactor is versatile and has also been used to grow and characterize biofilms of varying species.
This test method describes how to sample and analyze biofilm for living cells.
Advanced laboratories offer services for evaluating product efficacy in preventing or removing biofilms using in vitro techniques. This method represents environments where the resulting biofilm exists at a liquid interface under high shear conditions. It is used to model potable water systems, industrial water lines, oral biofilms, toilet bowls, washing machines, and other scenarios where biofilm binds more tightly to a surface.
The ASTM E2562-17 test method also describes how to analyze a biofilm for living cells recorded in colony forming units per surface area. This test method requires an in vitro laboratory to demonstrate how to grow, sample and analyze biofilms under high shear conditions.
Biofilm bacteria differ from hanging bacteria of the same genotype. Using biofilm growth reactors, biofilms are grown with certain properties. These properties and the biofilm are then modified by system parameters.
Within the scope of ASTM E2562-17 standard, our organization, with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it has given for businesses in various sectors, CDC also provides standard testing services for the quantification of pseudomonas aeruginosa biofilm grown with high shear and continuous flow using a biofilm reactor.
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