The ASTM E2799-17 standard published by the American Society for Testing and Materials (ASTM) describes the operational parameters required to grow and treat a pseudomonas aeruginosa biofilm in a high-throughput screening test known as the MBEC (minimum biofilm eradication concentration) physiology and genetics test. The equipment used in these tests consists of a plastic cap with 96 latches and a receiver plate corresponding to 200 individual wells with a maximum working volume of 96 µL. The biofilm is established on the pegs with gentle mixing under batch conditions, that is, with no nutrient flow into or out of a single well. The biofilm formed is transferred to a new recipient plate for disinfectant efficacy testing. The reactor design allows simultaneous testing of multiple disinfectants or a disinfectant with multiple concentrations and amplification of samples. In this way, the test becomes an efficient screening tool.
This test method includes the specific operational parameters required for growing a pseudomonas aeruginosa biofilm, but this device is versatile and used to grow, evaluate and study biofilms of different species.
This test method also includes verification of disinfectant neutralization. This test method describes how to sample biofilm and measure living cells.
Biofilm bacteria differ from hanging bacteria of the same genotype. Using biofilm growth reactors, biofilms are grown with certain properties. These properties and the biofilm are then modified by system parameters.
Briefly, with this test method, the susceptibility of the pseudomonas aeruginosa bacterial species to multiple test materials such as antibiotics or biocides is determined. The MBEC (minimum biofilm eradication concentration) test allows multiple test conditions to be run simultaneously and allows multiple concentrations of test materials to be evaluated at once.
Among the numerous test, measurement, analysis and evaluation studies it has given for businesses in various sectors, our organization, with its trained and expert staff and advanced technological equipment, is the standard test for testing the disinfectant effectiveness against pseudomonas aeruginosa biofilm by using the MBEC test within the scope of ASTM E2799-17 standard. also provides services.
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