Determination of Total Kjeldahl Nitrogen (SM 4500-Norg B Method)

Determination of Total Kjeldahl Nitrogen (SM 4500-Norg B Method)

Developed the SM 4500 Norg BB method, which includes a test method for the determination of total Kjeldahl nitrogen, within the scope of the National Environmental Methods Index (NEMI).is not. Kjeldahl methods (4500-Norg.B and C) determine nitrogen in the ternary state. It does not take into account nitrogen in the form of azide, azine, azo, hydrazone, nitrate, nitrite, nitrile, nitro, nitroso, oxime, and semi-carbazone. Kjeldahl nitrogen is the sum of organic nitrogen and ammonia nitrogen.

Determination of Total Kjeldahl Nitrogen (SM 4500-Norg B Method)

The most important factor affecting the choice of the macro or semi-micro Kjeldahl method for determining organic nitrogen is its concentration.

  • The Macro-Kjeldahl method is applicable to samples containing low or high concentrations of organic nitrogen, but requires a relatively large sample volume for low concentrations.
  • In the semi-micro-Kjeldahl method, which can be applied to samples containing high concentrations of organic nitrogen, the sample volume should be selected to contain organic plus ammonia nitrogen in the range of 0,2-2 mg.

The most reliable results are obtained with fresh samples. If immediate analysis is not possible, samples should be preserved for the Kjeldahl method by acidifying them with concentrated sulfuric acid at pH 1.5-2.0 and storing them at 4°C.

In the tests and analyzes carried out in our institution, which has a wide range of technological equipment, trained and experienced staff and applies the most modern methods, national and international standards and legal regulations in force are complied with. Among the numerous tests performed in this context, there are also services for the determination of total Kjeldahl nitrogen (SM 4500-Norg B method).

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