OECD procedure 428 is designed to provide information on the absorption of a test substance applied to the surface of a skin sample that separates the two chambers of a diffusion cell (a donor chamber and a recipient chamber). Both static and flow diffusion cells are accepted. In other words, this test method is designed to obtain information about the absorption of a test substance applied to excised skin.
There are two groups of methods for measuring skin absorption and dermal transmission: in vivo and in vitro methods. In vivo methods are well established and provide pharmacokinetic information in a variety of animal species. In vitro methods have also been used for many years to measure skin absorption.
Chemicals in work environments or other environments can come into contact with the skin and be absorbed. Determining the extent of skin absorption is an important step in the risk assessment of this type of chemical. Many factors can influence the numerical value used to determine the extent of skin absorption, such as exposure time, product formulation, dose, and skin fate of the chemical. While skin absorption studies are available for many pesticides, these studies have not been done for most other chemicals.
In vitro skin penetration studies using human skin are conducted to clearly assess the effect of a compound on the skin and provide data to assist decision and approval mechanisms for agrochemical, pharmaceutical and consumer products. In advanced laboratories, these studies are carried out in accordance with OECD Test Guideline 428. Animal hides, including farm animals, laboratory or domestic species, are also evaluated using these methods. These data are compared with human data for risk assessment or cross-species comparison requirements and used to design dermal studies.
Meanwhile, in vitro permeability testing is seen as a critical tool for understanding drug delivery into the various layers of the skin as part of the formulation selection process.
Among the numerous tests, measurements, analyses, and evaluations our organization provides for businesses in various sectors, we also offer in vitro testing services to determine skin absorption under OECD Procedure 428, utilizing a trained and expert staff and advanced technological equipment.
To get an appointment, to get more detailed information or to request an evaluation, you can ask us to fill in our form and reach you.
