The direct peptide reactivity test (DPRA) (OECD 442C) is a chemical test method that models the negative outcome pathway in skin sensitization, the first major event in protein reactivity with the skin. Compounds that cause allergic contact dermatitis are often electrophilic in nature (ie, reagents that attach to electrons). This test method identifies dermal sensitizers based on their reactivity with synthetic peptides containing nucleophilic amino acid residues lysine and cysteine. Using liquid chromatography - ultraviolet, the conjugation of the test material with peptides is analyzed. In advanced laboratories, these tests are performed in accordance with good laboratory practice as well as OECD 442C test guideline. On the other hand, DPRA is performed in conjunction with other in vitro methods to evaluate the skin sensitization potential of various chemicals. This test method is compatible with a range of solvent systems and is more economical than animal testing.
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Addressing the downstream pathway switch event on OECD procedure 442C covalent binding to proteins, this method includes three different test methods based on measuring covalent binding to peptides:
The first two methods are designed for hazard identification, and the third method is designed for the identification of strong sensitizers.
Skin sensitization is defined by the United Nations global harmonized chemical classification and labeling system as an allergic reaction following skin contact with a tested chemical. The test described here is at the regulatory level for hazard identification of skin sensitizers and non-sensitizers. the ayes have it.
The test methods in question comply with the cosmetic directive no. 1223/2009 and a number of legal regulations in force in the European Union countries. It was also included in the registration, evaluation, authorization and restriction of chemicals directive (REACH) in 2016. Data generated using the DPRA method are evaluated in the context of an integrated approach, ie in combination with other in vitro and chemical skin sensitization models.
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