The kinetic turbidimetric analysis method is the most widely used method for endotoxin detection and requires a photometric instrument to measure the turbidity change rate. Turbidimetry is an analytical and optical technique that serves biomedicine, microbiology and biotechnology research and is based on the appearance of solid particles in a homogeneous solution. This loss of homogeneity causes the light passing through the solution to not be of the same intensity as before the appearance of turbidity. Turbidity is the optical property of a sample that allows radiation to be dispersed and absorbed rather than transmitted in a straight line through the sample. If the transmitted light is measured at a certain wavelength, a value proportional to the concentration of the substance responsible for the turbidity or substances present in the solution is obtained. A spectrophotometer is used for these measurements. Special instruments called turbidimeters have been developed for this technique. These are different variations of a spectrophotometer.
Turbidimetric analysis methods are frequently used in biology, primarily to control cellular growth and the number of cells in solution, and for other analytical techniques related to the detection of microorganisms. In order to control the growth of a population of cells by turbidimetry, which can be, for example, bacteria, it is necessary to take into account that not only the number of cells, but also the size of these cells has an effect on the distribution of light. Turbidimetry allows to evaluate growth by estimating the cellular mass present in a sample. To avoid errors in turbidimetric measurements, normal curves are usually measured for each microorganism.
Turbidimetry has the advantage of being fast and non-destructive. Therefore, the determination of turbidity or optical density of liquid cultures is considered a widely used analytical tool to monitor the growth of pure bacterial cultures. In recent years, this measurement approach has been preferred more and more with the development of optical technology. Equipment including different light sources and detectors is used to measure the number of cells in different formats, from culture tubes to microplates. For example, recent advances in this field are based on the development of high-throughput assays and the study of microbial colonies, particularly applied to the field of food microbiology. The use of UV light sources to monitor bacterial growth is also increasing.
Our organization also provides kinetic turbidimetric analysis services with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.
To get an appointment, to get more detailed information or to request an evaluation, you can ask us to fill in our form and reach you.
