Dissolved organic carbon is defined as organic matter passing through a filter (filters usually range in size from 0,7 to 0,22 µm). In contrast, particulate organic carbon is carbon that is very large and is filtered through a sample. Straw, tea, or a body of water that appears brownish likely has a high organic carbon load. Dissolved and particulate organic carbon are important components in the carbon cycle and are primary food sources for aquatic food webs. Dissolved organic carbon changes aquatic ecosystem chemistry by contributing to acidification in low alkaline, weakly buffered, freshwater systems. It also forms complexes with trace metals, forming water-soluble complexes that are transported by organisms.
Dissolved organic carbon is measured by several different methods. High temperature combustion and ultraviolet/persulfate oxidation methods are widely used. The sample preparation protocol is the same for both methods. The sample is collected in a glass container by cooking at 550 degrees for 2-4 hours in the laboratory. The cooking process is to remove any residual carbon that may cause contamination in or on the collecting pan. The sample is then filtered through a glass filtration device. Commonly used filters include glass fiber filters, silver membrane filters or nitrocellulose/polypro filters. After the samples are taken, they are stored in the cold until processed. It is processed as soon as possible to prevent sample change after filtration.
The high-temperature combustion method to determine dissolved organic carbon involves converting the inorganic carbon into dissolved carbon dioxide and removing it from the sample. The remaining organic carbon is then oxidized to carbon dioxide at a high temperature, which is detected by the device's non-dispersive infrared sensor.
The ultraviolet/persulfate oxidation method combines the sample with an acid, reducing the sample's pH to 2.0. This process converts the inorganic carbon into dissolved carbon dioxide and is then cleared from the sample. A persulfate reagent is then added to the sample, and the remaining carbon is oxidized by UV radiation to form carbon dioxide, which is detected by the device's non-dispersive infrared sensor and correlated with the total organic carbon content.
Our organization also provides organic carbon determination services with its trained and expert staff and advanced technological equipment, among the numerous test, measurement, analysis and evaluation studies it provides for businesses in various sectors.
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