
Having a sufficient concentration of dissolved oxygen is critical to maintaining the aquatic life and aesthetic quality of streams and lakes. Determining how organic matter affects the dissolved oxygen concentration in a stream or lake is an integral part of water quality management. The degradation of organic matter in water is measured as biochemical or chemical oxygen demand. Oxygen demand is a measure of the amount of oxidizable substances that reduce dissolved oxygen concentrations in a water sample.

In the biochemical oxygen demand test, there are two stages of decomposition: the carbonaceous stage and the nitrogenous stage. The carbonaceous stage is the first stage and represents this part of the oxygen requirement involved in the conversion of organic carbon to carbon dioxide. The nitrogenous stage is the second stage and represents the combined carbonaceous surplus nitrogen demand when organic nitrogen, ammonia and nitrite are converted to nitrate.
Briefly, biochemical oxygen demand (BOD) is a measure of the amount of oxygen required to remove waste organic materials from water during the decomposition process by aerobic bacteria (i.e. bacteria living only in an oxygen-containing environment). The change in dissolved oxygen concentration is measured in water samples at a given temperature over a period of time.
Biochemical oxygen demand testing is a bioassay technique that involves measuring the oxygen consumed by bacteria while stabilizing the organic matter in the waste, normally as in nature, but under normal laboratory conditions. Traditionally, the test is performed over a period of 20 days at 5 degrees Celsius, expressed as milligrams of oxygen consumed per liter of sample during incubation during this period. This value usually indicates the degree of organic pollution of the water.
Broadly speaking, biochemical oxygen demand (BOD) is a chemical method for determining the amount of dissolved oxygen required by aerobic biological organisms in a water body to decompose organic material present in a given water sample, at a specified temperature, over a specified period of time. is the procedure. Although this procedure is widely used as an indicator of the organic quality of water, it is not a definitive quantitative test.
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