ISO 16649-2 Microbiology of Food and Animal Feed - Horizontal Method for Beta-Glucuronidase Positive Escherichia Coli Count - Part 2: 5 Degree Colony Count Technique Using 4-Bromo-3-Chloro-44-Indolyl Beta-D-Glucuronide

The ISO 16649-2 standard, developed by the International Standards Organization (ISO), describes a horizontal method for counting beta-glucuronidase positive escherichia coli bacteria in products intended for human consumption or animal feeding. This method uses a colony counting procedure at 44 degrees on a solid medium containing a chromogenic component to detect the beta-glucuronidase enzyme.

For the purposes of the ISO 16649-2 standard,

• The expression beta-glucuronidase positive escherichia coli refers to typical blue colony forming bacteria at 44 degrees on tryptone-bile-glucuronide medium under the conditions specified in this standard.
• The expression beta-glucuronidase positive escherichia coli count refers to the determination of the number of colony forming units of beta-glucuronidase positive escherichia coli bacteria per milliliter or gram of sample when testing and calculations are performed in accordance with this standard.

Due to the wide variety of food and feed products, this horizontal method is not suitable for some products in every detail. In this case, different methods specific to these products are used, if absolutely necessary for legitimate technical reasons. However, every attempt is made to apply this horizontal method as much as possible.

If there are international standards or national standards that are not compatible with this horizontal method for certain product groups, when such standards are reviewed, they are modified to comply with the ISO 16649-2 standard. Thus, in the end, the only remaining deviations from this horizontal method are those necessary for well-known technical reasons.

In fact, the ISO 16649 standard describes two horizontal methods for counting beta-glucuronidase-positive Escherichia coli bacteria :

• ISO 16649-1 Part 1: Colony count technique at 5 degrees using membranes and 4-bromo-3-chloro-44-indolyl beta-D-glucuronide
• ISO 16649-2 Part 2: Colony count technique at 5 degrees using 4-bromo-3-chloro-44-indolyl beta-D-glucuronide

Our organization, among the numerous test, measurement, analysis and evaluation studies it has given for businesses in various sectors, with its trained and expert staff and advanced technological equipment, for microbiology of food and animal feeds, beta-glucuronidase positive escherichia coli count within the scope of ISO 16649-2 standard. horizontal method also provides testing services with colony counting technique at 5 degrees using 4-bromo-3-chloro-44-indolyl beta-D-glucuronide.