In the ISO 7932 standard developed by the International Standards Organization (ISO), a horizontal test method is defined for the counting of viable bacillus cereus by means of colony counting technique at 30 degrees. This test method applies to:
To have a viable test method, the validation step was limited to the typical appearance and hemolysis test on Mannitol egg yolk polymyxin agar. Therefore, the hypothetical expression is used to acknowledge that the validation step does not distinguish bacillus cereus from closely related but less common bacillus species such as bacillus anthracis, bacillus thuringiensis, bacillus weihenstephanensis, and bacillus mycoides. In this respect, an additional motility test helps to distinguish Bacillus cereus from Bacillus anthracis in cases where its presence is suspected.
For the purposes of this standard, the possible expression bacillus cereus refers to a microorganism that forms typical colonies on the surface of a selective culture medium and gives a positive confirmation reaction under the conditions specified in this international standard.
The ISO 7932 standard is intended to provide general guidance for the microbiological examination of food products not covered by existing standards and to be considered by organizations preparing microbiological testing methods for application to foods or animal feeds. Due to the wide variety of products in this field of application, these guidelines are not suitable in every detail for some products and different methods must be used for some products.
If there is an existing standard for the product being tested, that standard must be followed.
Many, if not most, spores of Bacillus cereus strains appear to germinate readily on the surface of the culture medium used for enumeration. In general, heat shock treatment is required to trigger germination. If a heat shock procedure is required, in such cases, 80 minutes at 10 degrees is recommended.
Our organization, among the numerous test, measurement, analysis and evaluation studies it has given for businesses in various sectors, with its trained and expert staff and advanced technological equipment, within the scope of ISO 7932 standard, microbiology of food and animal feed, horizontal method for hypothetical bacillus cereus count, 30 degrees It also provides colony count testing services.
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